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Widespread laundering of protected wildlife into legal supply-chains is a poorly understood, under-enforced threat in Latin America, with little empirical research existing. Wildlife laundering involves a blurring of legal and illegal enterprise – creating novel opportunities for financial-investigation approaches, should an enhanced evidence base allow. This first-of-its-kind project targets this issue, building the evidence base to strengthen financial-investigation approaches. By undertaking in-depth situational and case-study analyses, it informs innovative, evidence-based approaches to IWT in Colombia and Mexico, thereby reducing poverty.